Establishment of an optimal hypo-osmotic swelling test by examining single spermatozoa in four different hypo-osmotic solutions.

Pregnancy Outcome with Intrauterine Insemination in Patients with Unexplained Recurrent Abortion Whose Partners Have Abnormal Hypo-Osmotic Swelling Test

Background: Recurrent abortion is defined as three or more consecutive pregnancy losses. We aimed to determine the relationship between male sperm parameters and hypo-osmotic swelling score and recurrent abortion. We also studied whether washing of spermatozoa and intrauterine insemination in patients with recurrent miscarriage and low score hypo-osmotic swelling test in male partners could imp...

Evaluating the Functional Status of Spermatozoa in Smokers Using Hypo-osmotic Swelling Test

Development of the Best Hypo-osmotic Swelling Solution for Evaluation of Functional Membrane Integrity of Spermatozoa of Nili-ravi Buffalo Bull

The functional integrity of spermatozoa in various domestic animals like horse, cattle and swine is evaluated by hypo osmotic swelling test. The hypo osmotic swelling test has not been tested in the fresh semen of Nili-Ravi buffalo bull. The aim of the present study was evaluation of membrane integrity by establishing the best hypo osmotic swelling solution. Ten solutions with different osmolar...

SUCCESSFUL PREGNANCY OUTCOME WITH lUI IN PATIENTS WITH UNEXPLAINED RECURRENT MISCARRIAGE, WHOSE MALE PARTNERS HAVE LOW SCORE HYPO-OSMOTIC SWELLING TEST

In order to determine outcome of pregnancy with lUI in patients with unexplained recurrent miscarriage whose husbands have low hypo-osmotic swelling test scores, a prospective clinical intervention was performed at a university referral clinic of recurrent abortion. Out of 56 patients whose husbands had abnormal hypo-osmotic swelling tests, 43 patients underwent lUI, from which only 14 pat...

Time course of hypo-osmotic swellings of human spermatozoa: evidence of ordered transition between swelling subtypes.

The hypo-osmotic swelling test (HOST or HOS test) usually takes into consideration the total HOS response value with no emphasis either on the value of the response subtypes or the response evaluation time. This study investigated the time course of HOS responses and analysed their physiological relevance. Raw semen spermatozoa and Percoll washed spermatozoa were used in the experiment. The mor...